
heating element.(bulb,heating pad,etc.).ģ. This incubator will be used cultivate E.Coli.ġ. We made a simple incubator that maintains a temperature of 37 ☌ using materials that are easily available. They also need a circulation system for equal distribution of heat in the box. Coli cells is approximately 37 ☌, as these organisms grow well under such conditions. The most commonly used temperature both for bacteria such as the frequently used E. The simplest incubators are insulated boxes with an adjustable heater, typically going up to 60 to 65 ☌ (140 to 150 ☏), though some can go slightly higher (generally to no more than 100 ☌). Incubators are essential for a lot of experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial as well as eukaryotic cells. The incubator maintains optimal temperature of the atmosphere inside it.


Then first egg-crate with fan with is blowing Air on heat tape not into incubator. Pipe frame is going now directly on the floor. They accumulate way too much heat and then radiate it so temp was impossible to control.ĭifferences between thermostat reading and thermometer was reaching 3-4 degrees with was totally unacceptable. Ok Looks Like My Design Had few flaws so modifications was needed.įirst off Water directly on Heat element=Bad Idea. Special thanks for PghBall and his Tread: with was my inspiration. You have finished Incubator.Īll system is controlled by previous version on Herpstat and as backup I Have Accurite thermometer just as Control.Ĭost without thermostat 155$ give or take 5$.
#DIY INCUBATOR PLUS#
all around 7 $ in local Home Depot.Īnother piece of Egg-crate plus sensor from Thermostat and that basically all. Then as space breaker i uses 1 inch plastic pipe and 4 90 degrees connectors. I've Used standard low Speed computer case 12V fan with is connected to 12V Dc power Adapter from Dollar Store. Then we have piece of egg-crate You can but that in local Home depot or Lowes for around 12-15$ per 2x4' Piece.Įgg-crate is a base for ventilator. On Dry Run after 24 hours water in the bottle was 118.5 F temp in upper part of cooler was 109 F

it fit very nice 9 1liter Smart Water bottles. Reason i go with 100qt is that only size wide enough for 6qr boxes so i can go with 3 6 qt egg boxes wide-wise or 2 biter ones length-wise.Īs heat source I've used new 6 inches TGH Heat tape got it from RBI for 18$ with Connector. I've bought it from KMart for 67 and change delivered to my home.

Just Finished my new "baby" so Decided post a pictures and cost.Īs a base I used 100 qt Igloo max cold cooler.
